Text Box: Legionella Incidence Rates
The percentage isolation rates for water samples positive for Legionella during the 13 month period up until last month are shown in the following graph. The average isolation rate during the period was 10.5% (compared to 16.5% for the sample period in 2002/2003). Of the positive samples, 3.9% had a Legionella count in excess of 1,000 CFU/mL, 53.4% had a count in the range 100 to less than 1,000 CFU/mL and 42.8% had a count below 100 CFU/mL.
Text Box: Welcome to the latest edition of our newsletter BioTalk for the water testing and air-conditioning industry. This newsletter is produced four times a year with special editions for topical or current issues. There are regular items and general information on microbiological issues. Your feedback and suggestions are most welcome. Copies of this newsletter and previous issues are available from our web site. 
BioTalkBiotech
LaboratoriesNEWSLETTERWater/Airconditioning
Industry

 Biological Testing  Reg No. 2787

Cert No. 7176 – 10

Editor: Glen Pinna

Text Box: Recent Legionella Outbreaks
(Source: http://members.dodo.net.au/~jamgreen/legionnaires'_disease_2004.htm)

Sydney. Australia. March 2004. 
Two clusters of Legionnaires disease, totalling 11 people, have been identified in NSW. The cases have occurred since January 2004. The first cluster involves 5 cases of Legionella longbeachae infection in Illawarra and Sydney. All patients are aged over 60 years, 3 are men and 2 are women. Two cases have died. Four of the five cases reported using potting mix. The other cluster was caused by Legionella pneumophila, the source of the infection is unknown

Oklahoma City. USA. March 2004.
At least 13 people who stayed at an Oklahoma City hotel have tested positive for the bacteria that can cause Legionnaire's disease. Two people were hospitalized and more than 50 reported flu-like symptoms after staying at the Comfort Suites at Interstate 40 and Meridian in Oklahoma City. It is thought that the pool or hot tub was responsible. The symptoms reported by those who have become ill include a rapid onset of fever, chills, fatigue, headache and muscle aches, without pneumonia, leading officials to believe all of the cases are Pontiac fever.

Cobram. Australia. June 2004.
Four cases of Legionnaires' disease are being investigated in Cobram, Victoria. Three women and one man aged between 66 and 78 have been infected. The source has been identified as a cooling tower at the Murray Goulbourn dairy co-operative.

Legionellosis in Australia 2003
(Source: http://www1.health.gov.au/cda/Source/CDA-index.cfm)

The figures of legionellosis cases in Australia for the first 6 months of this year were 150 compared to 157 cases in for the same time period in 2003. The number of cases per State or Territory were: Victoria 46, Western Australia 22, South Australia 17, New South Wales 48. Queensland 12, Northern Territory 3, ACT and Tasmania both had 1 case.

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August 2004

 

Issue No.

Text Box: There is an internet newsgroup for Legionella established at http://groups.yahoo.com/group/legionnaires_disease/   I would request that all experts in water treatment and concerned people go to this site, join the group and visit it to ask and answer questions posted by others.
Text Box: The above graph shows the monthly Legionella isolation rates from water samples for the last three years. Further data can be obtained from our web site or contacting the Editor.
Text Box: Biotech Laboratories Pty Ltd
Level 2, Administration Building, Greenslopes Private Hospital, Newdegate Street, Greenslopes Qld  4120
PO  Box 505  Indooroopilly  Qld  4068    Ph: (07) 3847 9488     Facsimile: (07) 3847 9890
E-mail:  general@biotechlab.com.au     Web site: http://www.biotechlab.com.au/
Text Box: Pathogenic micro-organisms associated with water - Part 4

         A variety of micro-organisms that can cause illness have been found in water, these can be introduced into the body by drinking and inhalation, or by exposure to skin or mucous membrane. They include bacteria, protozoa, algae and viruses. This discussion will cover the description, significance, water treatment required for elimination and health significance where applicable.

 

         Pseudomonas aeruginosa

 

         No guideline value has been set for Pseudomonas aeruginosa in drinking water.

 

         Although Pseudomonas aeruginosa is commonly found in faeces, soil, water and sewage, it cannot be used as an indicator of faecal contamination, as in is not universally present in faecal matter and can also be found in many aquatic environments and on surfaces in contact with water. It’s presence can, however, be used to indicate poor general cleanliness in a water distribution system and the quality of bottled water. Pseudomonas aeruginosa can also be found in some foods.

 

         Pseudomonas aeruginosa is an opportunistic pathogen, meaning that it exploits some break in the host defences to initiate an infection.  It causes urinary tract infections, respiratory system infections, dermatitis, soft tissue infections, bacteremia, bone and joint infections, gastrointestinal infections and a variety of systemic infections, particularly in patients with severe burns and in cancer and AIDS patients who are immunosuppressed. Pseudomonas aeruginosa infection is a serious problem in patients hospitalized with cancer, cystic fibrosis, and burns. The case fatality rate in these patients is 50 percent.

 

         It is therefore obvious that contamination of hospital water supplies with high levels of Pseudomonas aeruginosa can be a serious situation, although a direct link between nosocomial infection and contaminated water supplies has yet to be conclusively proven.

 

         The control of Pseudomonas aeruginosa can generally be achieved by maintaining free available chlorine levels of 0.2 to 0.5 mg/L.

 

         It is commonly found in poorly disinfected swimming pools and spa pools, where cases of eye, ear and folliculitis (skin inflammation) have been reported. Although there are not guidelines for drinking water, acceptable limits in swimming and spa pools are generally less than one organism per 100mL. In bottled water, the levels are much more stringent and are less than one organism per 250mL.

 

 

         Despite the absence of drinking water acceptable limits, the presence of Pseudomonas aeruginosa in a water supply may indicate a serious deterioration in the overall bacterial quality. In these situations it is unlikely that Pseudomonas aeruginosa will be the only contaminating bacteria and numerous other bacteria groups are likely to be present. This will often be associated with a deterioration of other physical characteristics such as taste, odour and turbidity.

 

 

         The next BioTalk will deal with Salmonella.

 

Source: Australian Drinking Water Guidelines - 6: 1996. National Health and Medical Research Council of Australia & Agriculture and Resource Management Council of Australia and New Zealand.  Foodborne Microorganisms of Public Health Significance. 5th Edition. 1997. AIFST (NSW Branch Food Microbiology Group. And US. FDA Foodborne Pathogenic Microorganisms and Natural Toxins Handbook.